Coding

Part:BBa_J72022:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)


{Bmll44} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Overlap extension (Wobble Construction)

Wobble construction of Bmll44 {rbs_at_10000.myc>}

Wobble mll44/mll45                (102, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll44         {rbs_at_10000.myc>}
----
mll44    Forward wobble for {rbs_at_10000.myc>} (Bmll44)
CCATAGAATTCATGAGATCTACGCAATATCACACGGAACCGGAGGGCTAACTCGCATGTCTGAAC
mll45    Reverse wobble for {rbs_at_10000.myc>} (Bmll44)
CGTTAGGATCCCAGATCCTCTTCTGAGATGAGTTTTTGTTCAGACATGCGAGTTAG

References